Journal: Frontiers in Pharmacology

Article Title: Trifolin inhibits the calcium-driven contraction pathway in vascular smooth muscle

doi: 10.3389/fphar.2025.1573483

Figure Lengend Snippet: Trifolin suppressed the activation of CaM/MLCK/p-MLC2 pathway in Ang Ⅱ-infused hypertensive mice. (A–D) The protein expression of CaM, MLCK, MLC2, and p-MLC2 in abdominal aortas from each group was detected using immunohistochemistry. Representative images at ×400 magnification are shown. Scale bar = 50 μm. Data were presented as mean ± SD; * p < 0.05 vs. Control group, # p < 0.05 vs. Ang Ⅱ group.

Article Snippet: Antibodies against MLCK (Cat. No. 48846), p-MLC2 (Cat. No.11114), and GAPDH (Cat. No. 52902) were obtained from Signalway Antibody (College Park, MD, United States).

Techniques: Activation Assay, Expressing, Immunohistochemistry, Control

Journal: Frontiers in Pharmacology

Article Title: Trifolin inhibits the calcium-driven contraction pathway in vascular smooth muscle

doi: 10.3389/fphar.2025.1573483

Figure Lengend Snippet: Trifolin attenuated the increased expression of SOC channel and calcium signaling-related proteins in A7R5 cells. (A) The expression of STIM1 and ORAI1 protein in Ang Ⅱ-stimulated A7R5 Cells after trifolin treatment was determined by western blot analysis, and GAPDH was used as the internal control. (B) The expression of CaM, MLCK, MLC2, and p-MLC2 proteins in Ang Ⅱ-stimulated A7R5 cells after trifolin treatment was determined by western blot analysis, and GAPDH was used as the internal control. Data were presented as mean ± SD; * p < 0.05 vs. the Control group, # p < 0.05 vs. the Ang Ⅱ group.

Article Snippet: Antibodies against MLCK (Cat. No. 48846), p-MLC2 (Cat. No.11114), and GAPDH (Cat. No. 52902) were obtained from Signalway Antibody (College Park, MD, United States).

Techniques: Expressing, Western Blot, Control